Journal: Cell Transplantation

Article Title: Early and Dose-Dependent Xenogeneic Mesenchymal Stem Cell Therapy Improved Outcomes in Acute Respiratory Distress Syndrome Rodent Through Ameliorating Inflammation, Oxidative Stress, and Immune Reaction

doi: 10.1177/09636897231190178

Figure Lengend Snippet: Impact of HUCDMSCs on downregulating oxidative signaling in rat PECL2 underwent lung extraction treatment. (A) Protein expression of NOX-1* versus other groups with different symbols (†, ‡), P < 0.001. (B) Protein expression of NOX-2* versus other groups with different symbols (†, ‡), P < 0.001. (C) Protein expression of apoptosis signal-regulating kinase 1 (ASK1)* versus other groups with different symbols (†, ‡), P < 0.001. (D) Protein expression of mitogen-activated protein kinase kinase 4 (p-MKK4)* versus other groups with different symbols (†, ‡), P < 0.001. (E) Protein expression of p-MKK7* versus other groups with different symbols (†, ‡), P < 0.001. (F) Protein expression of phosphorylated c-Jun N-terminal kinases 1/2 (p-JNK1/2)* versus other groups with different symbols (†, ‡), P < 0.001. (G) Protein expression of phosphorylated cellular Jun (p-c-JUN)* versus other groups with different symbols (†, ‡), P < 0.001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( n = 3). Symbols (*, †, ‡) indicate significance (at 0.05 level). ANOVA: analysis of variance; ARDS: acute respiratory distress syndrome; HUCDMSCs: human umbilical cord–derived mesenchymal stem cells; PECL2: pulmonary epithelial cell line L2.

Article Snippet: The membranes were incubated with the indicated primary antibodies [tumor necrosis factor (TNF)-α (1:1000, Cell Signaling), interleukin (IL)-1β (1:1000, Cell Signaling), NADPH oxidase (NOX)-1 (1:1500, Sigma), NOX-2 (1:500, Sigma), mitochondrial Bax (1:1000, Abcam), cleaved poly (ADP-ribose) polymerase (PARP) (1:1000, Cell Signaling), caspase 3 (1:1000, Cell Signaling), phosphorylated (p)-Smad3 (1:1000, Cell Signaling), transforming growth factor (TGF)-β (1:1000, Abcam), cytosolic-cytochrome-C (1:5000, BD), high-mobility group box 1 (HMGB-1) (1:1000, Cell Signaling), toll-like receptor (TLR)-2 (1:1000, Abcam), TLR-4 (1:1000, Novus), myelin and lymphocyte (MAL) (1:1000, Abcam), translocating chain-associated membrane (TRAM) (1:1000, Invitrogen), myeloid differentiation primary response 88 (MYD88) (1:1000, Abcam), TIR-domain-containing adapter-inducing interferon-β (TRIF) (1: 1000, Abcam), TNF receptor associated factor 6 (TRAF6) (1:1000, Abcam), IkB-β (1:1000, Abcam), p-nuclear factor (NF)-κB (1:1000, Abcam), apoptosis signal-regulating kinase 1 (ASK1) (1:1000, Abcam), phosphorylated (p)-mitogen-activated protein kinase kinase 4 (MKK4) (1:1000, Cell Signaling), p-MMK7 (1:1000, Invitrogen), c-Jun N-terminal kinase (JNK) (1: 1000, Abcam), p-JUN (1:1000, Abcam), cyclophilin D (1:1000, Abcam), dynamin-related protein 1 (Drp1) (1:1000, Cell Signaling)] for 1 h at room temperature.

Techniques: Extraction, Expressing, Comparison, Derivative Assay

Journal: Cell Transplantation

Article Title: Early and Dose-Dependent Xenogeneic Mesenchymal Stem Cell Therapy Improved Outcomes in Acute Respiratory Distress Syndrome Rodent Through Ameliorating Inflammation, Oxidative Stress, and Immune Reaction

doi: 10.1177/09636897231190178

Figure Lengend Snippet: Protein expression of oxidative cell stress signaling in lung parenchyma by day 5 after ARDS induction. (A) Protein expression of NOX-1* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (B) Protein expression of apoptosis signal-regulating kinase 1 (ASK1)* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (C) Protein expression of NOX-2* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (D) Protein expression of mitogen-activated protein kinase kinase 4 (p-MKK4)* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (E) Protein expression of p-MKK7* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (F) Protein expression of phosphorylated c-Jun N-terminal kinases 1/2 (p-JNK1/2)* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. (G) Protein expression of phosphorylated cellular Jun (p-cJUN)* versus other groups with different symbols (†, ‡, §, ¶), P < 0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( n = 6). Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level). ANOVA: analysis of variance; ARDS: acute respiratory distress syndrome; HUCDMSCs: human umbilical cord–derived mesenchymal stem cells; HUCDMSC 3h : HUCDMSCs administration at 3 h after 48 h ARDS induction; HUCDMSC 24h : HUCDMSCs administration at 24 h after 48 h ARDS induction; HUCDMSC 3h/24h : HUCDMSCs administration at 3 h and 24 h after 48 h ARDS induction; SC: sham control.

Article Snippet: The membranes were incubated with the indicated primary antibodies [tumor necrosis factor (TNF)-α (1:1000, Cell Signaling), interleukin (IL)-1β (1:1000, Cell Signaling), NADPH oxidase (NOX)-1 (1:1500, Sigma), NOX-2 (1:500, Sigma), mitochondrial Bax (1:1000, Abcam), cleaved poly (ADP-ribose) polymerase (PARP) (1:1000, Cell Signaling), caspase 3 (1:1000, Cell Signaling), phosphorylated (p)-Smad3 (1:1000, Cell Signaling), transforming growth factor (TGF)-β (1:1000, Abcam), cytosolic-cytochrome-C (1:5000, BD), high-mobility group box 1 (HMGB-1) (1:1000, Cell Signaling), toll-like receptor (TLR)-2 (1:1000, Abcam), TLR-4 (1:1000, Novus), myelin and lymphocyte (MAL) (1:1000, Abcam), translocating chain-associated membrane (TRAM) (1:1000, Invitrogen), myeloid differentiation primary response 88 (MYD88) (1:1000, Abcam), TIR-domain-containing adapter-inducing interferon-β (TRIF) (1: 1000, Abcam), TNF receptor associated factor 6 (TRAF6) (1:1000, Abcam), IkB-β (1:1000, Abcam), p-nuclear factor (NF)-κB (1:1000, Abcam), apoptosis signal-regulating kinase 1 (ASK1) (1:1000, Abcam), phosphorylated (p)-mitogen-activated protein kinase kinase 4 (MKK4) (1:1000, Cell Signaling), p-MMK7 (1:1000, Invitrogen), c-Jun N-terminal kinase (JNK) (1: 1000, Abcam), p-JUN (1:1000, Abcam), cyclophilin D (1:1000, Abcam), dynamin-related protein 1 (Drp1) (1:1000, Cell Signaling)] for 1 h at room temperature.

Techniques: Expressing, Comparison, Derivative Assay